Cryo-electron tomography provides 3D snapshots of proteins at work within their functional cellular environments, allowing users to see and understand how they, and...

By applying new correlative fluorescence light microscopy (fLM) and electron cryotomography (ECT)...

John Briggs' group is studying the structure and the molecular assembly mechanisms of enveloped viruses such as HIV and influenza virus...

Electron cryotomography (ECT, also known as cryo-electron tomography, cryo-ET) is a 3D imaging technique that can analyze the structure of complex and varied biological assemblies in their native...

Cryo-electron tomography (CET) is an emerging imaging modality that is suitable for molecular and cellular structural studies. CET allows obtaining three-dimensional (3D)...

Cryo-electron tomography (cryo-ET) is an electron microscopy technique that allows macromolecular structures to be studied at a resolution of approximately 4 nm and in 3D...

Cryo-tomography of transmissible gastroenteritis coronavirus (TGEV; CoV group 1) reveals a similar virion organization, suggesting a general model for the architecture of...

Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in...

Observe biological specimens in their native environment at cryo-temperatures. Architecture and host interface of environmental chlamydiae revealed by electron cryotomography.

Cellular tomography. Revealing the molecular organization of cells in a physiologically Cellular processes, such as cell adhesion and mitosis, can be investigated by cryo-ET.

Electron cryotomography. Last updated March 29, 2019. This schematic shows the concept of electron tomography. A sample is imaged in a TEM as it is tilted to different angles, resulting in a...

Electron cryotomography (CryoET) is an imaging technique used to produce high-resolution (~1-4 nm) three-dimensional views of samples, typically biological macromolecules and cells.[1][2]...

Cryo-tomography of intact, vitrified cells provides a three dimensional view of their structure and organization in a snapshot of the living state.

We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

Cryo-electron tomography (cryoET) can provide 3D reconstructions, or tomograms, of pleomorphic objects such as organelles or cells in their close-to-native states.

...cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography.

Cryo-electron tomography (cryo-ET or electron cryotomography) is a type of electron cryomicroscopy where tomography is used to obtain a 3D reconstruction of a sample from tilted 2D...

Cryo-Electron Tomography data analysis. In situ structure determination by subtomogram analysis. Averaging and classification of sub-tomograms. We aim to establish single...

Electron cryotomography (ECT) is an emerging technology that allows thin samples such as macromolecular complexes and small Electron cryotomography. Cold Spring Harb Perspect Biol.

Step 5: 3D Cryo Tomography. Leica Microsystems and Thermo Fisher Scientific have collaborated to develop a fully integrated Cryo Electron Tomography Workflow.

Cryo-electron tomography (cryo-ET) enables the 3D visualization of cellular organization in near-native state which plays important roles in the field of structural cell biology.

Cryo-electron tomography (cryo-ET) offers this opportunity, and in this chapter we provide an overview of recent advances in sample preparation, data acquisition and data...

Cryo‐electron tomography (cryo‐ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures.

Cryo-electron tomography (cryo-ET) can give unprecedented insight into these complexes in the context of their natural environment. However, the application of...

Electron tomography is used to reveal the structure of cells in three dimensions (3D). You are going to email the following Three-dimensional deconvolution processing for STEM cryotomography.

Cryo-electron tomography — (cryo ET or electron cryotomography) is a type of electron cryomicroscopy where tomography is used to obtain a 3D reconstruction of a sample from tilted 2D...

Cryo-EM and Cryo-Electron Tomography. Master. Content. One of the greatest advantages of cryo-EM relative to conventional structural biology techniques is its ability...

Using cryo-electron microscopy and tomography, we performed a comprehensive inventory of human ejaculate EVs. The sample was neither centrifuged, fixed...